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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become noticed in lots of tumors, which leads to chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was uncovered to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial position in advertising mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two supplemental splice variants have been claimed but specific nucleotide sequence information isn't but readily available.
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Blocking peptides are peptides that bind particularly for the target antibody and block antibody binding. These peptide ordinarily is made up of the epitope recognized through the antibody.
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This manual outlines additional achievable optimization of cellular and immunoassay parameters to make sure the best possible final results are...
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This tutorial outlines even more feasible optimization of mobile and immunoassay parameters to make sure the best possible benefits are...
Antibodies bound to the blocking peptide not bind to the epitope about the concentrate on protein. By comparing the staining through the blocked antibody vs . the antibody alone, one can see which staining is precise.
Concentrate on Details Cyclin E belongs on the really conserved cyclin family members, whose associates are characterized by a remarkable periodicity in protein abundance throughout the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins show distinctive expression and degradation styles which lead on the temporal coordination of every mitotic function. Cyclin E kinds a complex with and functions as being a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary which is degraded as cells progress via S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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